AccuNanoBead


Product Descriptions

Magnetic beads have important applications in the purification of biosimilar protein and antibody drugs. Magnetic beads have many advantages over existing resins and are better suited for automation of the purification process. The principle of magnetic beads are coating with appropriate functional group in the surface of magnetic beads. With the function group, magnetic beads can combine protein and then using external magnetic field, magnetic beads is moved for isolation.


The Features of Magnetic Nanobeads

Average Sizes of Magnetic Nanobeads are 400 nm
Uniform Disperse Size Distribution of Magnetic Nanobeads
Sphere Form of Ni-NTA coated Magnetic Nanobeads
Excellent Efficiency of Protein Purification
Rapid Purification Method using Magnetic Nanobeads.


Applications of Magnetic Beads

Magnetic Protein Purification
Magnetic Biosimilar Separation
Magnetic Antibody Separation



Figure 1. FE-SEM image of Magnetic Nanobeads; SEM images show that Magnetic Nanobeads are sphere shape.



Figure 2. EDS analysis result of Magnetic Nanobeads; EDS shows that Magnetic Nanobeads are composed of silica and Iron oxide.



Figure 3. Size distribution diagram of silica magnetic nanobeads. The average size of magnetic beads is approximately 400 nm.



Protein Purification Method by using Magnetic Nanobeads

First, load the protein sample to tube and then put Magnetic Nanobeads to combine protein sample together with Magnetic Nanobeads. Fix Magnetic Nanobeads with external Magnetic field to wash the solution and then elute protein from Magnetic Nanobeads. Using Magnetic Nanobeads to use in purification is much faster to separate protein compare to an existing method.

Figure 4. Recombinant Protein and Antibody protein purification protocol by Magnetic Nanobeads



Figure 5. (a) SEM image of 400 nm Bioneer magnetic nanobeads, (b) SEM image of A company magnetic beads, (c) SEM image of B company magnetic beads, (d) SEM image of C company magnetic beads, (e) SEM image of D company magnetic beads
Figure 5 shows scanning electron microscope images of Bioneer magnetic nanobeads and different company magnetic beads(b~e). Scanning electron microscope image of synthesized magnetic nanobeads shows that magnetic nanobeads of Bioneer is 400 nm size and spherical shape. And different company magnetic beads are micro meter size and various shape.


Comparison of Protein Purification Yield

The protein purification yields of Bioneer Ni-NTA magnetic nanobeads and other company magnetic beads are listed in Table 1. These results indicate that the purification yield of Bioneer Magnetic beads is higher than that of four different company magnetic beads. In addition, purity of protein purification was more than 90%. His-Tagged DNA Polymerase protein of the 97 kDa expressed in E. coli was used in experiment. Purification yield is amount of purified target protein from loading protein. Bioneer magnetic nanobeads represents the highest purification yield compared with that of four different company magnetic beads because the surface area of magnetic beads is increased due to the size effect.

Table 1. Comparison of protein purification yield of bioneer beads and other company beads


Figure 6. Comparison of elution protein of bioneer beads and other company beads.



Figure 7. SDS-PAGE image of purified 94 kDa DNA Polymerase with Bioneer Magnetic Nano Beads.


AccuNanoBead

Magnetic Nanobeads have potential in the research and separation media for DNA/RNA, protein and cell isolation. Package sizes of magnetic nanobeads are 1 g, 5 g, 10 g and bulk package


Ordering Information

Cat No. Product Description Pack size List Price(US$)
TA-1010-1 AccuNanoBeadTM Silica Magnetic Nanobeads, size 400nm 0.5 g/25 ml 250.00
TA-1010-2 AccuNanoBeadTM Silica Magnetic Nanobeads, size 400 nm 1.0 g/50 ml 450.00
TA-1010-3 AccuNanoBeadTM Silica Magnetic Nanobeads, size 400 nm 10 g/500 ml 2,550.00
TA-1011-1 AccuNanoBeadTM NH2 Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 280.00
TA-1012-1 AccuNanoBeadTM COOH Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 290.00
TA-1013-1 AccuNanoBeadTM Epoxy Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 310.00
TA-1014-1 AccuNanoBeadTM C18 Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 310.00
TA-1015-1 AccuNanoBeadTM Streptavidin Magnetic Nanobeads, size 400 nm 50 mg/25 ml 250.00
TA-1016-1 AccuNanoBeadTM Biotin Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 310.00
TA-1017-1 AccuNanoBeadTM Ni-NTA Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 510.00
TA-1019-1 AccuNanoBeadTM Thiol Magnetic Nanobeads, size 400 nm 0.5 g/25 ml 380.00
TA-1021-1 AccuNanoBeadTM Protein G Magnetic Nanobeads, size 400nm 40mg/1ml 152.00
TA-1021-2 AccuNanoBeadTM Protein G Magnetic Nanobeads, size 400nm 200mg/10ml 522.00
TA-1021-3 AccuNanoBeadTM Protein G Magnetic Nanobeads, size 400nm 500mg/25ml 990.00
TA-1022-1 AccuNanoBeadTM Protein A Magnetic NanoBeads, size 400 nm 40mg/1ml 152.00
TA-1022-2 AccuNanoBeadTM Protein A Magnetic NanoBeads, size 400 nm 200mg/10ml 522.00
TA-1022-3 AccuNanoBeadTM Protein A Magnetic NanoBeads, size 400 nm 500mg/25ml 990.00
TS-1000-1 Magnet Φ15x1.5 and Screw Tube 1.5 ml 1 set 5.00
*Please inquiry for bulk orders.


Related Products

Cat No. Product Description Pack size List Price(US$)
K-7250 AccuRapid™ Cell-Free Protein Expression Kit 45 ul x 24 reactions 450.00
K-7300 ExiProgen™ EC Protein Synthesis Kit 16 reactions 590.00
K-7301 ExiProgen™ EC Protein Synthesis Kit 32 reactions 990.00
K-7302 ExiProgen™ EC Protein Synthesis Kit 96 reactions 2,450.00
D-2010 AccuLadder™ Protein Size Marker (Broad) 500 ul 120.00
D-2020 AccuLadder™ Protein Size Marker (Low) 500 ul 70.00
K-3030 AccuPrep® Plasmid Extraction kit 200 prep 120.00
K-3034 AccuPrep® PCR purification Kit 200 prep 135.00
K-7210 ExiPrep™ His-tagged Protein Purification Kit 96 reactions 500.00
K-7200 AccuPrep™ His-tagged Protein Purification Kit - 210.00


• Manual_AccuNanoBead™ Streptavidin Magnetic NanoBeads

Brochure-1 [download]
Brochure-2 [download]
Brochure-3 [download]


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